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Marine Biodiversity of Raja Ampat Islands Project

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Training on Genetic Analysis and Bioinformatic
08 February 2017 09:08 AM

This course covers basic bioinformatics and genetic data analysis training for publication (two days). This course will help ensure the effective operation of the MEGA, Arlequin, Network, DnaSP Genetic Data Analyzer for both sequences of Genebank and own data, to help maximize throughput and obtain results for publication at journal. The course is designed for any individual who will be an operator of the MEGA, Arlequin, Network, DnaSP Genetic Data Analyzer. Please see the brochure for further details.
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PCR Product “Clean-up”: SAP/EXO
07 July 2016 20:41 PM

SAP stands for Shrimp Alkaline Phosphatase whereas EXO stands for Exonuclease I. SAP and EXO are two hydrolytic enzymes. The purpose of this procedure is used to chew up excess primers and remove excess dNTPs from your PCR product. This procedure is necessary to ensure clean and readable DNA sequences.
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Polymerase chain reaction (PCR)
07 July 2016 20:40 PM

PCR is an in vitro technique which allows the amplification of a specific DNA region that lies between two region of known DNA sequence. PCR is a biochemistry and molecular biology technique for isolating and exponentially amplifying a fragment or sequence of interest of DNA, via enzymatic replication, without using a living organism. It can be performed without restrictions on the form of DNA, and it can be extensively modified to perform a wide array of genetic manipulations. PCR was invented in 1983 by Kary Mullis. PCR is now a common technique used in medical and biological research labs for a variety of tasks, such as the sequencing of genes and the diagnosis of hereditary diseases, the identification of genetic fingerprints (used in forensics and paternity testing), the detection and diagnosis of infectious diseases, and the creation of transgenic organisms. PCR is being also used for biosystematics, population biology, conservation biology, ecology, developmental biology, and genetics.
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Previously on Articles

  • 07/06/2016 DNA Extraction DNA extraction is a routine procedure to collect D...
  • 07/05/2016 Electrophoresis Electrophoresis is a technique used in the laborat...
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