PCR Product “Clean-up”: SAP/EXO
The SAP/EXO protocol is the simplest way to clean up PCR products before sequencing. Exonuclease I removes residual single-sranded primers and any e This procedure is necessary to ensure clean and readable DNA sequences. xtraneous single-stranded DNA produced in the PCR. Shrimp alkaline phosphatase removes the remaining dNTPs from the PCR mixture.
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